TY - JOUR

T1 - SDS-PAGE for Myosin Heavy Chains: Fast and Furious

AU - Gerzen, O. P.

AU - Potoskueva, Iu. K.

AU - Permyakova, Yu. V.

AU - Grebenschchikova, A. V.

AU - Selezneva, I. S.

AU - Nikitina, L. V.

N1 - This work was implemented using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology within the Governmental assignment to the Institute of Immunology and Physiology of the Russian Academy of Sciences; theme reg. no. 122022200089-4.

PY - 2022

Y1 - 2022

N2 - Using our own new modification of one-dimensional sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) we successfullyseparated myosin heavy chains from different chambers of rat heart.The key features of modification are changes in the content andbuffer composition of the separating gel, and employment of constantcurrent instead of voltage. These changes allow carrying out a saferand faster one-dimensional SDS-PAGE for cardiac myosin heavy chainsusing significantly fewer reagents.

AB - Using our own new modification of one-dimensional sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) we successfullyseparated myosin heavy chains from different chambers of rat heart.The key features of modification are changes in the content andbuffer composition of the separating gel, and employment of constantcurrent instead of voltage. These changes allow carrying out a saferand faster one-dimensional SDS-PAGE for cardiac myosin heavy chainsusing significantly fewer reagents.

UR - https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=tsmetrics&SrcApp=tsm_test&DestApp=WOS_CPL&DestLinkType=FullRecord&KeyUT=000935467900010

U2 - 10.1134/S0022093022070109

DO - 10.1134/S0022093022070109

M3 - Article

VL - 58

SP - S92-S97

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - S1

ER -