Research output: Contribution to journal › Article › peer-review
Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - SDS-PAGE for Myosin Heavy Chains: Fast and Furious
AU - Gerzen, O. P.
AU - Potoskueva, Iu. K.
AU - Permyakova, Yu. V.
AU - Grebenschchikova, A. V.
AU - Selezneva, I. S.
AU - Nikitina, L. V.
N1 - This work was implemented using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology within the Governmental assignment to the Institute of Immunology and Physiology of the Russian Academy of Sciences; theme reg. no. 122022200089-4.
PY - 2022
Y1 - 2022
N2 - Using our own new modification of one-dimensional sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) we successfullyseparated myosin heavy chains from different chambers of rat heart.The key features of modification are changes in the content andbuffer composition of the separating gel, and employment of constantcurrent instead of voltage. These changes allow carrying out a saferand faster one-dimensional SDS-PAGE for cardiac myosin heavy chainsusing significantly fewer reagents.
AB - Using our own new modification of one-dimensional sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) we successfullyseparated myosin heavy chains from different chambers of rat heart.The key features of modification are changes in the content andbuffer composition of the separating gel, and employment of constantcurrent instead of voltage. These changes allow carrying out a saferand faster one-dimensional SDS-PAGE for cardiac myosin heavy chainsusing significantly fewer reagents.
UR - https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=tsmetrics&SrcApp=tsm_test&DestApp=WOS_CPL&DestLinkType=FullRecord&KeyUT=000935467900010
U2 - 10.1134/S0022093022070109
DO - 10.1134/S0022093022070109
M3 - Article
VL - 58
SP - S92-S97
JO - Journal of Evolutionary Biochemistry and Physiology
JF - Journal of Evolutionary Biochemistry and Physiology
SN - 0022-0930
IS - S1
ER -
ID: 35452168