TY - JOUR

T1 - Biomimetic materials based on hydroxyapatite patterns for studying extracellular cell communication

AU - Zyrianova, Polina I.

AU - Eltantawy, Mervat M.

AU - Silin, Danil V.

AU - Korolev, Ilya S.

AU - Nikolaev, Konstantin

AU - Kozodaev, Dmitry A.

AU - Slautina, Alla

AU - Surmenev, Roman

AU - Kholkin, Andrei

AU - Ulasevich, Sviatlana

AU - Skorb, Ekaterina

N1 - Authors acknowledge RSF grant no. 21-13-00403 for the financial support of the part for the fabrication of electrodes with polymer buffer layers, functional hydrogel, and RSF grant no. 19-79-10244 for the financial support of the part with cells experiments. Priority 2030 Program is acknowledged for infrastructural support. Part of the work (PFM measurements and their development of their methodology) was financially supported by the Ministry of Science and Higher Education (grant agreement # 075-15-2021-588 of 1 June 2021). A.S.S. is grateful to S. Salmiyarov for his contribution PFM date analysis. The equipment of the Ural Center for Shared Use “Modern nanotechnology” Ural Federal University was used.

PY - 2024/2/1

Y1 - 2024/2/1

N2 - The study of cellular ion channels forms a basic understanding of healthy organ functioning and the body as a whole; however, the native role of signal transmission through ion channels between cells remains unclear. The success of the signal transmission investigation depends on the methods and materials used. Therefore, it is necessary to develop a new approach and system for studying detecting cell–cell communication. In this work, we suggest the system of hydroxyapatite patterns demonstrating piezoresponse in conjunction with fiber-based biosensors for detection of electrical signaling in cellular communities. Our system does not disrupt the integrity of cell membrane. The cells are located on self-assembled hydroxyapatite patterns forming the tissue patterns and communicating via spatially propagating waves of calcium, sodium, and potassium ions. These waves result from positive feedback caused by the activation of Ca2+ channels. The fiber-based ion-selective microelectrodes fixed above the patterns are used to detect the sodium, potassium, calcium ion currents in the extracellular space. We use norepinephrine to activate the Ca2+ channels result in intracellular Ca2+ release between the cell communities on different patterns. This system could be perspective as an efficient platform to lab-on-a-chip study as well as fundamental understanding of cellular communication during regeneration.

AB - The study of cellular ion channels forms a basic understanding of healthy organ functioning and the body as a whole; however, the native role of signal transmission through ion channels between cells remains unclear. The success of the signal transmission investigation depends on the methods and materials used. Therefore, it is necessary to develop a new approach and system for studying detecting cell–cell communication. In this work, we suggest the system of hydroxyapatite patterns demonstrating piezoresponse in conjunction with fiber-based biosensors for detection of electrical signaling in cellular communities. Our system does not disrupt the integrity of cell membrane. The cells are located on self-assembled hydroxyapatite patterns forming the tissue patterns and communicating via spatially propagating waves of calcium, sodium, and potassium ions. These waves result from positive feedback caused by the activation of Ca2+ channels. The fiber-based ion-selective microelectrodes fixed above the patterns are used to detect the sodium, potassium, calcium ion currents in the extracellular space. We use norepinephrine to activate the Ca2+ channels result in intracellular Ca2+ release between the cell communities on different patterns. This system could be perspective as an efficient platform to lab-on-a-chip study as well as fundamental understanding of cellular communication during regeneration.

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U2 - 10.1016/j.matdes.2024.112718

DO - 10.1016/j.matdes.2024.112718

M3 - Article

VL - 238

JO - Materials and Design

JF - Materials and Design

SN - 0264-1275

M1 - 112718

ER -