We studied the biological activity of water and ethanol extracts of basidiomycetes of the species Armillaria spp., A. borealis, Russula delica, Lactarius deliciosus, Cantharellus cibarius, Leccinum scabrum, L. aurantiacum. Were used to study the isolated metabolites the recommended methods for determining the quantitative analysis. During the work, it was found that different solvents are suitable for different groups of substances. Amino acids are better extracted into ethanol, phenols, flavonoids and sugars into water.