Our study aimed to characterize the acid-base balance in order to evaluate hematological parameters and take them into account when evaluating the biophysiological state of the organism in order to prevent energy consumption. The acid-base balance of mature specimens of moored frog (Rana. arvalis) was studied. living in the Kalinovskie cuts, the South-Western Forest Park (Forest Park Zone) and Lake Kurtan, (Kurgan Region) in Yekaterinburg. Where the pH and Where the concentration of ctCO2 was studied, pCO2, concentration of bicarbonate ions сHCO3-, the concentration of blood buffer systems (excess of bases in whole blood— BE), (concentration of buffer bases— BB),(excess of deficit of standard blood bases— cSBE),hematocrit Hct,hemoglobin ctHb, Na+, K+ and Ca+2 in plasma. Significant differences in one of the blood parameters cNa, cK, cCa, Hct, cHCO3, cBE, cBB and ctCO2 in the compared samples of animals, in both habitats were revealed in the study. where the concentration of ctCO2 increased in the blood of frogs living in the Kalinovsky cuts and Southwest, compared with samples collected from Kurtan. This increase increased cHCO3-, cBE, cBB and Ca+2 levels. While the concentration of Na+, K+ and ctHb in the blood of frogs in Kurtan increased. In addition, statistically significant differences were observed in concentrations cHCO3,cBE and ctCO2 between morphs (Maculatus and Striata) for the Moored Frog.