The development of Epsilon-near-zero (ENZ) metamaterial connected to a microwave (mm-Wave) bio-sensor, which exhibits very high practical performance, ultrafast response, and high accuracy, has become critical and benchmark in the integrated on-chip devices known as lab on chip (LoC). In order to achieve high efficiencies in perfect absorber based on harnessing the input mm-waves, a simple slot-antenna-based configuration is introduced, which involves efficient dielectric-metal-dielectric (DMD) configuration containing guided mode resonances and plasmonic induced transparency (PIT). By capitalizing on the simple configuration DMD and two slots antenna consisting of two bright modes and strong interaction between them, a new type of optical sensor is generated, which is extremely effective in harnessing input electromagnetic waves. Our research not only focuses on the design and fabrication of a mm-wave bio-sensor based on double-PIT (D-PIT) phenomena using ENZ metamaterial, but also explores the feasibility assessment of non-invasive application in order to achieve the biological materials of the human body, including bad bacteria of the bowel. The novel D-PIT-based sensor has garnered significant attention due to its exceptional capabilities and innovation in various practical optical sensing fields, particularly in the detection of biological materials. By tuning the dimensions of the slots antenna, we are able to achieve the first and second bright modes and static tunability of the proposed metamaterial sensor. Furthermore, the interaction between surface plasmon polaritons (SPPs) and input light demonstrates transverse electrical (TE) surface modes, which are insensitive to external factors.