TY - JOUR

T1 - Effects of Antihistamines on Behavioral Activity of Zebrafish Danio rerio

AU - Zhdanov, A. V.

AU - Komelkova, M. V.

AU - Gorbunova, M. A.

AU - Khatsko, S. L.

AU - Sarapultsev, A. P.

AU - Kalueff, A. v.

PY - 2023/11/1

Y1 - 2023/11/1

N2 - Histamine receptors play a pivotal role in various physiological functions (ranging from allergic responses to memory and sleep regulation), hence representing important drug targets. While second-generation antihistamines have successfully been used in rodents and humans, testing their effects in non-traditional animal models furthers our understanding of their physiological activity and facilitates the development of novel drug candidates. Here, we examined the effects of the first-generation antihistaminic drug chloropyramine and the second-generation drugs loratadine and cetirizine, at concentrations of 1, 5, and 10 mg/L, on adult zebrafish behavior in the novel tank test. All three drugs significantly altered zebrafish locomotor activity, decreasing the distance traveled and average velocity, as well as increasing low acceleration frequency. Chloropyramine (5 and 10 mg/L) and loratadine (1, 5, and 10 mg/L) also significantly reduced top entries compared to the control. Additionally, chloropyramine (5 mg/L) increased the total duration of top entries, whereas loratadine (10 mg/L) reduced this behavior compared to controls. Overall, chloropyramine and loratadine exhibited a sedative effect typical of antihistamines, whereas cetirizine solely reduced locomotor activity without affecting other patterns of fish behavior. Thus, cetirizine demonstrated the least side effects on the central nervous system among the studied drugs, making it the optimal and safest choice among antihistamines.

AB - Histamine receptors play a pivotal role in various physiological functions (ranging from allergic responses to memory and sleep regulation), hence representing important drug targets. While second-generation antihistamines have successfully been used in rodents and humans, testing their effects in non-traditional animal models furthers our understanding of their physiological activity and facilitates the development of novel drug candidates. Here, we examined the effects of the first-generation antihistaminic drug chloropyramine and the second-generation drugs loratadine and cetirizine, at concentrations of 1, 5, and 10 mg/L, on adult zebrafish behavior in the novel tank test. All three drugs significantly altered zebrafish locomotor activity, decreasing the distance traveled and average velocity, as well as increasing low acceleration frequency. Chloropyramine (5 and 10 mg/L) and loratadine (1, 5, and 10 mg/L) also significantly reduced top entries compared to the control. Additionally, chloropyramine (5 mg/L) increased the total duration of top entries, whereas loratadine (10 mg/L) reduced this behavior compared to controls. Overall, chloropyramine and loratadine exhibited a sedative effect typical of antihistamines, whereas cetirizine solely reduced locomotor activity without affecting other patterns of fish behavior. Thus, cetirizine demonstrated the least side effects on the central nervous system among the studied drugs, making it the optimal and safest choice among antihistamines.

UR - https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=tsmetrics&SrcApp=tsm_test&DestApp=WOS_CPL&DestLinkType=FullRecord&KeyUT=001137643100030

UR - https://elibrary.ru/item.asp?id=64604625

U2 - 10.1134/S0022093023060303

DO - 10.1134/S0022093023060303

M3 - Article

VL - 59

SP - 2297

EP - 2303

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - 6

ER -