The assumption of transformation of soil seed banks under the influence of invasive plants was verified. For this purpose, the taxonomic wealth and the number of seedlings from the soil seed bank in the thickets of the invasive maple tree ( Acer negundo L.) were estimated. In the vegetation experiment seedlings emerged from seeds on soils selected in two types of habitats in Ekaterinburg (in dense thickets of A. negundo and in geomorphologically and edaphically similar habitats, but without A. negundo ) were used. In addition, seedlings on sod-podzolic soil were analyzed from suburban meadow. A slight negative influence of A. negundo on the abundance of seedlings from the soil seed bank was established. In the version with an ash-leaved maple, the number of shoots was 1.5-2.5 times lesser than in urban habitats without A. negundo. The taxa wealth of seedlings differed only between suburban and urban habitats, regardless of whether the urban habitats were transformed due to the growth of invasive ash-leaved maple.