Objective. To assess the characteristics of stress perception and response in healthcare facility workers at different stages of the COVID-19 pandemic. Subjects and methods. An online survey of healthcare workers was conducted using the Perceived Stress Scale-10 (PSS-10), the Hospital Anxiety and Depression Scale (HADS), and the Subjective Asthenia Rating Scale. Results. The investigators identified a significant number of workers with non-constructive perception and response to stress factors during the pandemic. Female workers having up to f ive years’ work experience, who provided assistance to patients with coronavirus infection and/or had experienced this disease, were burnout risk groups. The severity of affective disorders and asthenic manifestations was higher in COVID-19 convalescent healthcare workers than in healthy individuals. Conclusion. The investigation has yielded real-time data on the psychoemotional status of healthcare workers during the COVID-19 pandemic and identified potential risk groups in developing burnout syndrome, which primarily require professional psychological assistance.