The aim of the study is to identify differences in the species composition of weeds in spring rapeseed crops in two geographically remote regions of the Russian Federation - Lipetsk (Central black earth region) and Sverdlovsk (Ural region). The analysis was carried out using methods of comparative Floristics and distribution of species by classes of constancy of occurrence. Differences between segetal weed flora elements in the agrophytocenoses of canola compare regions, as in floral structure, consisting of the first two "triads" of the floristic spectra and confirmed the low rate coefficient of species similarity. Species differences are represented by species that pollute rapeseed crops only in one of the compared regions. Among the species common to the agrophytocenoses of rapeseed in the two regions are such species as, Chenopodium album L., Echinochloa crusgalli (L.) Beauv., Silene praténsis (Rafn) Godr., Euphorbia helioscopia L, Fallopia convolvulus (L.) A. Loeve, Viola arvensis Murr. they are characterized by higher rates of occurrence in the Lipetsk region, and Capsella bursa-pastoris (L.) Medik., Cirsium setosum (Willd.) Bess, Erodium cicutarium (L.) L. Her., Thlaspi arvense L. - in the Sverdlovsk region. The dominant species in the Lipetsk region are Raphanus raphanistrum L., Chenopodium album L., Echinochloa crusgalli (L.) Beauv., Silene praténsis (Rafn) Godr., and in the Sverdlovsk region - Galium vaillantii DC., Stellaria media (L.) Vill., Capsella bursa-pastoris (L.) Medik., Thlaspi arvense L., Chenopodium album L., Cirsium setosum (Willd.) Bess. To protect rapeseed crops from weeds in different regions that differ in terms of heat and moisture availability, it is necessary to develop regional protection systems, preceded by mandatory monitoring studies.